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KMID : 1143420160090380755
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Public Health Weekly Report
2016 Volume.9 No. 38 p.755 ~ p.759
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Development of Viral Vector Using Attenuated Vaccinia Virus Strain, KVAC103
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Lee Yoon-Jung
Son Ho-Sun
Yeo Sang-Gu
Yoo Jung-Sik
Lee Sang-Won
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Abstract
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BACKGROUND: KVAC103 is a highly attenuated vaccinia virus strain, developed for third-generation smallpox vaccine at the Korean National Institute of Health (KNIH). It is created through multiple propagations and plaque isolation of conventional smallpox vaccine strain of vaccinia virus in Vero cells. It has a 19.5-kb deletion in the left wing of the genome and can replicate only in restricted mammalian cell lines. Using KVAC103, we constructed a recombinant viral vector for preventive and therapeutic vaccines.
METHODOLOGY: To improve the replication of the virus and develop efficient recombinant virus production methods, the plasmid shuttle vectors, containing C7L or K1L, were constructed and tested for recombinant virus production.
RESULTS: Using the plasmid shuttle vectors containing these genes, the recombinant viruses from KVAC103 strain were easily obtained. The recombinant viruses replicated efficiently in diverse mammalian cell lines and expressed higher levels of GFP antigens.
CONCLUSION: Recombinant KVAC103 vaccinia virus can be used as a unique viral vector to overcome the difficulties in developing vaccines against some infectious diseases and cancers by inducing effective humoral and cellular immune responses. Additionally, this method may be used for rapid vaccine development against threatening outbreaks
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KEYWORD
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